We are particularly interested in understanding how Notch signalling regulates several distinct processes during pancreatic development. The majority of our studies are done with chicken, mice and embryonic stem cells. We use a wide variety of techniques including gene targeting, transgenic mice, gene arrays, immunohistochemistry, confocal microscopy, tissue explants, and in ovo electroporation. The aim of all our experiments is to understand the genetic and cellular interactions that direct pancreatic organogenesis.
We are currently mapping the temporal requirement of Notch signalling in different processes occurring during pancreatic development as well as determining the cell biological details of Notch signalling events. We are also involved in a collaborative tool-generating project that aims to develop new fluorescent reporters for a number of developmental signalling pathways. These tools will significantly improve our ability to study signalling events in vivo and in vitro.