Ditlev Egeskov Brodersen
Department of Molecular Biology and Genetics - Structural Biology, University of Aarhus
Microbial survival mechanisms
We are interested in uncovering fundamental, molecular mechanisms underlying central survival mechanisms in both bacteria and archaea with the aim of understanding how microorganisms respond to nutritional stress, antibiotics, and other changes in their environment. Knowledge of these mechanisms can be used to develop new antimicrobial drugs. We use classical biochemistry as well as hybrid methods in structural biology (x-ray crystallography, electron microscopy, and small-angle x-ray scattering) in our work.
1) Mechanism of activation and activity of bacterial toxin-antitoxin systems (VapBC, RelBE, HicAB etc.), including the mechanism of RNA cleavage.
2) Understanding how phosphonate compounds are utilised as sources of phosphorous via the carbon-phosphorus lyase pathway in E. coli.
3)Understanding the interplay between toxin-antitoxin systems and CRISPR-Cas immunity in bacteria and archaea.
4)Regulation of (p)ppGpp synthesis and the effects of the alarmone during stress in bacteria.