Brage Storstein Andresen
Department of Biochemistry and Molecular Biology, University of Southern Denmark
The research in the BA laboratory has for many years focused on the molecular genetics and the molecular pathology of human disease with a special emphasis on regulation of normal and aberrant alternative pre-mRNA splicing. Initially, the research was mainly focused on studies of how mutations can disrupt the splicing regulatory mechanisms and cause disease. We have characterized numerous splicing regulatory elements and their binding proteins by using splicing reporter mini genes, expression systems for splicing regulatory factors and in vitro techniques.
In recent years, our laboratory has focused intensely on development of techniques for Next Generation Sequencing (NGS) of RNA, development of data-analysis pipelines, as well as global mapping and characterization of in vivo binding sites for splicing regulatory factors using iCLIP and eCLIP techniques.